| 1. |  YOGSCAST Kim | 6,564,771 | TACOMA: Compliance [ENDING] #5 | 38:44 |
| 2. |  Shenpai | 1,377,728 | An Easily Frightened Person Plays Alien: Isolation | 28:56 |
| 3. |  Gab Smolders | 1,157,659 | I beat Alien: Isolation on NIGHTMARE MODE | 1:10:12 |
| 4. |  Girls Play | 901,732 | EENY MEENY MINY CLOSET | Girls Play | Mortem | 12:15 |
| 5. |  Shizuka Rin Official | 653,382 | ありえん５ | 6:57:25 |
| 6. | TheRPGMinx | 270,864 | MAIZE + ALIENS ISOLATION! | 2:04 |
| 7. | Clare Siobhán | 141,773 | MY SCREAM BROKE THE MICROPHONE! - Alien Isolation - Survival Mode | 8:13 |
| 8. |  Meghan Yeah | 111,041 | Alien: Isolation - Part 6 - Walkthrough / Playthrough / Let's Play | 5:39:30 |
| 9. |  8 Bites | 108,130 | ALIEN ISOLATION BONUS EPISODE - Last Survivor | 20:23 |
| 10. | 39daph vods | 103,519 | 39daph Plays Alien: Isolation - Part 2 (Final) | 7:41:48 |
| 11. | Lavinia | 49,686 | The End -Ep 41 Let's Play: Alien Isolation | 22:26 |
| 12. | PressHeartToContinue | 43,793 | ALIEN ISOLATION HIGHLIGHTS: Spoopy Stream! | 9:35 |
| 13. | Lomadiah | 39,021 | Hannah & Nilesy - Star Trek #11 - Plank Spock! | 14:56 |
| 14. | Eneija | 33,548 | Let's Play Alien: Isolation EP55 // STAIRWAY TO HAPPINESS [FINALE] | 26:41 |
| 15. | 有栖マナAlice mana | 30,899 | 【Alien: Isolation】#5 そろそろ終わらせてください | 4:44:53 |
| 16. |  Jana Gamer | 25,817 | BARATAS GIGANTES - JOGO DE TERROR - The Roach Apartment | 5:08 |
| 17. | Liera | 24,252 | ENDING - Playing Alien Isolation For the First Time l Hard Mode - Pt.14 | 59:36 |
| 18. | Geek Remix | 24,160 | ENDING - Womz Play Observations Part 6 | 55:29 |
| 19. |  Loserfruit | 17,070 | ALIEN ISOLATION SCREAM HIGHLIGHTS! | 3:04 |
| 20. |  Caiyth | 16,814 | CAN'T FIND THE BUTTON - ALIEN ISOLATION #17 | 12:00 |
| 21. | KatFTWynn | 12,097 | Mother & Daughter Play: Alien Isolation: Crew Expendables DLC - ALIEN LOVES US ~Full Playthrough~ | 45:59 |
| 22. | Liara Gaming | 11,432 | Running from the androids in Alien Isolation #shorts | 0:53 |
| 23. | InquisitorMaster | 11,111 | ONE MINUTE SPEEDRUN!! | Alien Isolation Survival Mode | 4:14 |
| 24. | Eversor Fatalis | 10,565 | FEET | 0:43 |
| 25. | GamerJoob | 7,631 | Alien Isolation Part: 8 Finale (Spooky with Jade) | 25:43 |
| 26. | GamerKat09 | 6,202 | (P4) Let's Play - Perception [BLIND] - Surrounded by Dolls | 1:24:20 |
| 27. | Dandifying Gaming | 5,677 | Alien: Isolation | Reaction Compilation | 16:11 |
| 28. | Ashley Mariee | 5,521 | Alien: Isolation | THE BEGINNING | Ep 1 w/ AshleyMariee | 15:42 |
| 29. | GirlFromAus | 4,950 | Alien Isolation Playthrough Part 11 - An Insane Ending | 1:18:39 |
| 30. | More2Me 4Life | 4,115 | Chat Controls Me | Alien Isolation Congregation Lite Modded | 10:26:35 |
| 31. | Angel Alyx | 3,686 | HAPPY LATE HALLOWEEN | Alien Isolation LIVE | 3:40:04 |
| 32. |  Phenrir Mailoki | 3,303 | 🔴Super Maratona di Halloween - 2 di 4 (Slender, Alien Isolation, FNAF, Darker Skies) | 3:59:54 |
| 33. | Fiaura The Tank Girl | 3,123 | Observer (1) - Cyberpunk Meets Alien Isolation | 1:09:42 |
| 34. | OneRandomDolly | 2,947 | Alien Pen Drawing: Live Stream Alien Isolation | 3:01 |
| 35. |  Hai Halo Epel! | 2,480 | Senam Jantung Tidak Sehat【Alien: Isolation ft. @ReynardBlanc 】 | 2:58:35 |
| 36. | Connie K | 2,456 | Alien: Isolation | So we meet... | Part 3 | Connie Kuroi | 24:17 |
| 37. | My Cinnamon Phoe | 1,650 | Phoephoe FAILS to play Alien: Isolation Nightmare - I give up | 4:20 |
| 38. | Nerdlette Gaming | 1,579 | Alien: Isolation | Laney vs Alien Part 5 | 3:43:16 |
| 39. | NotSoProKitty | 1,364 | I've learnt so much about myself! | 0:38 |
| 40. | Celina VODs | 1,351 | Will I Survive This? - Alien: Isolation (Full Playthrough Part 6) | 6:12:01 |
| 41. | GamerGirls | 1,218 | Alien Isolation Episode 44 - Back to the Beginning | 34:43 |
| 42. | Virgo4u | 1,178 | Alien Isolation | Part 23 | OH BOY!!! | 18:00 |
| 43. | DoubleDamsels | 1,136 | Double Damsels Play | Alien: Isolation | Part 8 | Two Weeks From Retirement | 10:23 |
| 44. | Kureha Kurono 黒乃くれは | 1,023 | 【 Horror 】Alien Isolation【 悲鳴 】#Vuber #JPVtuber | 3:18:56 |
| 45. | WTFSexyHeadphones | 754 | SOMA #15 Lost, confused, and tired of your shit! | 32:10 |
| 46. |  AyinMaiden | 718 | My Alien Isolation Twitch Clips Compilation | 7:53 |
| 47. |  KITSUNEKON | 610 | 《Alien: Isolation / 異形：孤立》和最喜歡的異形一起玩躲貓貓！【KITSUNEKON｜Gaming】 | 2:08:00 |
| 48. | lilly waters | 550 | Get'er Done Lets Play: Alien Isolation Part 38 reloaded | 19:06 |
| 49. | Nashtya Newb | 536 | Nash survives to the end in ALIEN: ISOLATION | Final | 57:35 |
| 50. |  BRM | 517 | Умный Чужой, хакеры и медитация | Alien: Isolation | Good MOMents | 20:57 |
| 51. | A Gallows | 488 | AN UPDATE! Don't You Love My Updates? | 4:26 |
| 52. | AssassinGlasgow | 428 | A Couple Let's Plays: Alien Isolation | ALIENS, ANDROIDS, NOOOOO [Pt. 3] | 15:04 |
| 53. | MsCarsonelle | 395 | Let's Try Alien Isolation (Starring My Brother!) | 22:16 |
| 54. | Unbealievable | 368 | Livestream Archive \\ Let's Play Alien: Isolation w/ My Girlfriend Janome \\ STOP BREATHING #3 | 1:32:16 |
| 55. | Riona Aquaria 『retro ♡ mantique』 | 230 | Alien Isolation - I WILL BE BRAVE THIS TIME. I WILL SHOW YOU GUYS I'M AFRAID OF NOTHING | 5:56:28 |
| 56. | DarkRubberNeck | 228 | I think I may have self destructed my pc lol - Alien Isolation Last Survivor - 02 | 14:32 |
| 57. | spookibree | 147 | livestreaming to convince myself not to quit youtube | 41:42 |
| 58. | くのいち子のちゃんねる | 146 | 【VISAGE #4】くのいち子は叫ばない2021 feat.ウパルマン殿(2021.10.11） | 2:59:33 |
| 59. | KTris Ch. | 118 | Twitch Stream- Alien Isolation (Full Edited Ver.) | 31:52 |
| 60. | Usagisquared Plays | 108 | Usagisquared Plays Alien: Isolation - Ep 32 | 29:41 |
| 61. | Tani | 107 | 👨🏻🚀Alien Isolation🔥 #09 Mit Feuer gegen das Alien | 3:09:51 |
| 62. | Classy Katie | 77 | So dead! | 0:40 |
| 63. | mcrKirei | 42 | Alien: Isolation | Stream 2/21/16 | 2:38:28 |
| 64. | Mena Angelfire | 21 | Halloween Nights Day 17 Alien Isolation By Lady Amena | 27:30 |
| 65. | KrimsonKitty | 14 | Couch Co-op with Mucs and Alien Isolation VR! Sub goal reward stream! | 3:43:02 |
| 66. | Nyxira | 14 | Something's Wrong with Emma!! | OBSERVATION | 4:18:54 |
